Composite

Part:BBa_K3596050:Design

Designed by: Xinyu Liu   Group: iGEM20_GreatBay_SZ   (2020-10-22)


BIOT RBS mut3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2637
    Illegal PstI site found at 8227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 8227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2227
    Illegal BglII site found at 2691
    Illegal BglII site found at 4492
    Illegal BamHI site found at 907
    Illegal BamHI site found at 3016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2637
    Illegal PstI site found at 8227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2637
    Illegal PstI site found at 8227
    Illegal NgoMIV site found at 1134
    Illegal NgoMIV site found at 5979
    Illegal AgeI site found at 980
    Illegal AgeI site found at 1273
    Illegal AgeI site found at 1836
    Illegal AgeI site found at 4460
    Illegal AgeI site found at 6748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7754
    Illegal SapI.rc site found at 4096


Design Notes

no


Source

E. coli O157:H7, G. sulfurreducens

References